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. 2015 Oct 19;37(1):52–64. doi: 10.1002/humu.22909

Figure 1.

Figure 1

Identification of primary AR target genes in DUCaP cells. ChIP‐seq and gene expression microarray data obtained from androgen‐stimulated DUCaP cells formed the basis to identify genomic AR target sites (ARBSs) and the androgen‐regulated transcriptome of DUCaP cells. A: Validation of ChIP‐seq data. Schematic illustration of AR target sites showing ARBSs in the regulatory regions of prototype androgen‐responsive genes, KLK3 (PSA), FKBP5, and TMPRSS2, identified by ChIP‐seq. B: Venn diagram illustrations of androgen‐regulated genes following 8 and 24 hr of hormone (1 nM R1881) treatment in DUCaP cells. C: Venn diagram identifying genes that harbor ARBSs and are androgen regulated in the same direction in DUCaP cells stimulated for 8 or 24 hr. These 1,490 genes were designated as the primary AR target genes. D: Genes regulated by androgen stimulation at both time points in LNCaP cells are shown as overlaps. E: Comparison of androgen‐regulated genes in DUCaP with those in LNCaP cells.