scCO2-PAA inactivation of wet and dry PS533 (wild-type) B. subtilis spores, and by PAA alone in water. PS533 (wild-type) spores were treated for different times at 35°C with several levels of PAA from NovaKill™ either alone, in liquid with scCO2 or dry with scCO2, and spore viability was measured as described in Methods. The symbols for the different treatment conditions are: □, 50 ppm PAA alone; △, 150 ppm PAA alone; and ○, 300 ppm PAA alone; and scCO2 plus: ◇, 1 ml NovaKill™ Gen2 reagent with wet spores; ■, 1 ml NovaKill™ Gen2 reagent with dry spores; ▲, 8 ml NovaKill™ Gen2 reagent with dry spores; and ●, 16 ml NovaKill™ Gen2 reagent with dry spores.