Fig. 6A,B.

Proteins released from germinated untreated and scCO₂-PAA-treated spores. Untreated wet spores of strain PS533 (wild-type) and wet spores inactivated ~ 90% by scCO₂-PAA were heat activated, germinated, the supernatant fluid and pellet fractions from the germinated spores isolated and processed as described in Methods. Aliquots of processed A) germination supernatant fluid and B) spore pellets were run on SDS-PAGE, and the gels were stained and photographed as described in Methods. In A) the samples run in the various lanes were: a, 20 μl untreated spores; b, 5 μl untreated spores; c, mol wt markers (sizes in kDa just above the appropriate bands in panel A); d, 20 μl scCO₂-PAA treated spores; and e, 5 μl scCO₂-PAA-treated spores. In B) the samples run in the various lanes were: a, 10 μl untreated spores; b, 3 μl untreated spores; c, mol wt markers (sizes in kDa just above the same bands in panel A); d, 10 μl scCO₂-PAA treated spores; and e, 3 μl scCO₂-PAA-treated spores.