Table 2.
Mutations in untreated and scCO2-PAA treated spores*
| Numbers of mutants | ||||
|---|---|---|---|---|
| Spores treated | Inactivation - % | aux | spo | aux spo |
| PS533 (wild-type) | ||||
| Wet untreated | 0 | 0 | 0 | 0 |
| Dry treated | 90 | 1 | 1 | 0 |
| Dry treated | 99.5 | 0 | 0 | 0 |
| Wet treated | 92 | 0 | 0 | 0 |
| Wet treated | 99 | 0 | 0 | 0 |
| PS578 (α−β−) | ||||
| Untreated | 0 | 1 | 1 | 0 |
| Dry treated | 99.5 | 13 | 15 | 3 |
*
In each experiment colonies from 416 individual PS533 (wild-type) or PS578 (α−β−) spores either untreated or scCO2-PAA treated, were screened for auxotrophic (aux) or asporogenous (spo) mutants as described in Methods.