Table 3.
Effect of high salt +/− glucose on the viability of untreated or scCO2-PAA-treated spores*
Spore viability on various plates | ||||
---|---|---|---|---|
Spores treated | Inactivation (%) | LB1 | LB+NaCl | LB+NaCl+glucose |
Dry untreated | 0 | 100 | 130 | 120 |
Wet treated | 0 | 100 | 60 | 70 |
Dry treated | 95 | 100 | 2 | 20 |
Wet treated | 99 | 100 | 1.5 | 40 |
Various dilutions of untreated or scCO2-PA-treated PS533 (wild-type) spores were spotted in duplicate on normal LB medium (0.15 mol l−1 NaCl) plates with kanamycin (10 mg l−1) and with either 1 mol l−1 NaCl, or 1 mol l−1 NaCl plus 50 mmol l−1 glucose. Plates were incubated at 37°C for 16-72 h, and colonies were counted.
The viability of each spore preparation on normal LB medium plates was defined as 100%, and was measured as described in Methods.