Table 3.

Effect of high salt +/− glucose on the viability of untreated or scCO₂-PAA-treated spores^*

	Spore viability on various plates
Spores treated	Inactivation (%)	LB1	LB+NaCl	LB+NaCl+glucose
Dry untreated	0	100	130	120
Wet treated	0	100	60	70
Dry treated	95	100	2	20
Wet treated	99	100	1.5	40

^*Various dilutions of untreated or scCO₂-PA-treated PS533 (wild-type) spores were spotted in duplicate on normal LB medium (0.15 mol l⁻¹ NaCl) plates with kanamycin (10 mg l⁻¹) and with either 1 mol l⁻¹ NaCl, or 1 mol l⁻¹ NaCl plus 50 mmol l⁻¹ glucose. Plates were incubated at 37°C for 16-72 h, and colonies were counted.

¹The viability of each spore preparation on normal LB medium plates was defined as 100%, and was measured as described in Methods.