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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: J Appl Microbiol. 2015 Dec 9;120(1):57–69. doi: 10.1111/jam.12995

Table 3.

Effect of high salt +/− glucose on the viability of untreated or scCO2-PAA-treated spores*

Spore viability on various plates
Spores treated Inactivation (%) LB1 LB+NaCl LB+NaCl+glucose
Dry untreated 0 100 130 120
Wet treated 0 100 60 70
Dry treated 95 100 2 20
Wet treated 99 100 1.5 40
*

Various dilutions of untreated or scCO2-PA-treated PS533 (wild-type) spores were spotted in duplicate on normal LB medium (0.15 mol l−1 NaCl) plates with kanamycin (10 mg l−1) and with either 1 mol l−1 NaCl, or 1 mol l−1 NaCl plus 50 mmol l−1 glucose. Plates were incubated at 37°C for 16-72 h, and colonies were counted.

1

The viability of each spore preparation on normal LB medium plates was defined as 100%, and was measured as described in Methods.