Table 4.

Levels of various types of BacLight staining of different spore preparations^*

BacLight staining:	Poorly stained	Bright red	Bright green	Germination
Spore preparation	% of spores examined
Untreated dormant (327)†	100	0	0	-
Untreated germinated (509)	6	3	91	94
Treated wet – dormant (242)	96	4‡	0	-
Treated wet– germinated (472)	31	63	6	69§
Treated dry – dormant (380)	95	5‡	0	-
Treated dry – germinated (890)	78	22	0.5	22§
Autoclaved – dormant (370)	3	97	0.4	-

^*Spores in the images shown in Fig. 5a-f (and data not shown) were scored for weak staining (see panel a), bright red staining (see panel c), or bright green staining (see panel d). The spores treated wet were inactivated 90%, and the spores treated dry were inactivated 99%.

^†Values in parentheses are the numbers of individual spores scored.

^‡These spores generally exhibited an intermediate red staining intensity.

^§These values have not been corrected for any treated dormant spores that exhibited moderate red staining intensity, so may be lower than values shown.