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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: FEBS J. 2015 Oct 22;282(24):4643–4657. doi: 10.1111/febs.13541

Fig. 1.

Fig. 1

Mapping the NN-T20-NITN segment interacting with gp120. (A) Overlay of the 1H-15N-HSQC spectrum of free 15N-labeled NN-T20-NITN ( black) and in the presence of 1:0.5 molar ratio of mutgp120core (red). (B) Normalized residual intensity for NN-T20-NITN amide-protons cross-peaks upon addition of a 50% molar ratio of gp120 variants or BSA. The intensity of the 1H-15N cross peak of a given residue when the peptide is in the presence of gp120 or BSA was divided by the intensity of the same residue in free NN-T20-NITN. The cross peak exhibiting the highest intensity ratio was used for normalization The solid black line represents a threshold of 0.3 for the normalized residual intensity below which the change in cross peak intensity is considered significant. All spectra were recorded for 40 µM concentration of NN-T20-NITN at 298 K in 20 mM D11-Tris-HCl pH 7.6, 50 mM NaCl and 95% H2O/5% D2O.