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PCR conditions for sequencing analysis of the C22orf2 gene
| Exon | Primers (5'-3') | Annealing temperature (°C) | PCR product (bp) |
| 2 | ex2F: GGCATAAGGTCAGTGATCCAG | 58 | 236 |
| ex2R: TTCAGAGACACGCCTCAGCAC | |||
| 3–4 | ex3F: GGTCATATTGTTGGCGGAAG | 58 | 490 |
| ex4R: CCAAACTGGCATTGAGGAGA | |||
| 5 | ex5F: CCAGCAGCATCAGAGAAGTG | 56 | 245 |
| ex5R: AAAAGCACTCAGCCACATCC |
