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. Author manuscript; available in PMC: 2017 Jan 12.
Published in final edited form as: Cell Metab. 2015 Nov 25;23(1):143–154. doi: 10.1016/j.cmet.2015.10.014

Figure 6. Overexpression of tim enhances fat metabolism, which mediates lifespan extension.

Figure 6

(A) Overexpression of tim in whole body increases relative abundance of tim mRNA and circadian amplitude of tim expression. The data are normalized to the trough (ZT4) levels seen in control flies on AL diet. Values are mean ± SEM of 3 independent preparations. (B) Overexpression of tim in whole body increases triglyceride turnover upon AL. Values are mean ± SEM of 4 independent preparations. Statistical significance was determined using Student’s t-test and is denoted by ***p< 0.001, **p<0.01, and *p<0.05. (C) Fat body specific overexpression of tim increases triglyceride synthesis specifically in the abdomen and thorax of the flies on AL diet. Values are mean ± SEM of 4–5 independent preparations. Statistical significance was determined using Student’s t-test and *denotes p<0.05. (D) Co-expression of tim overexpression and ACC RNAi in whole body abrogates the AL dependent increase in survival. Kaplan Meier survival analysis of female flies with tim overexpression under AL (with RU486, red) conditions, control flies (without RU486, blue), ACC RNAi (with RU486, green) and tim overexpression with ACC RNAi (with RU486, black). (E) The mean lifespan observed in survival curves shown in (6D). Statistical analysis of the survival curves, complete genotype and number of flies are provided in Table S2 and for an independent repeat see Figure S6. (F) Levels of trilauryl glycerol (TLG) are increased in flies overexpressing tim and are reduced upon ACC RNAi in whole body on AL diet. Values are mean ± SEM of 4 independent preparations. Statistical significance was determined using Student’s t-test and is denoted by *p<0.05, **p<0.01, ***p<0.001.