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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: J Thromb Haemost. 2015 Dec 11;14(1):153–166. doi: 10.1111/jth.13181

Figure 8. TF+ tumor microvesicles induce accumulation of platelets in the mouse lung.

Figure 8

(A) C57Bl6J mice were treated with an infrared-labeled anti-mouse GPIX antibody (0.125 μg/g). Mice were then injected intravenously with BxPc-3 microvesicles (MVs) at 5, 30, and 60 minutes (9 μg total) or the same volume of PBS control. Mice were sacrificed 5 minutes after the last injection, the lungs were removed and platelet deposition imaged on an Odyssey Infrared imaging system. Quantification of lung fluorescence was performed on ImageJ software. *P<0.0001, n=4 by unpaired student’s t-test. (B) Representative lung images are shown from mice injected with MVs or PBS. Some mice would start to gasp and then die within minutes of TMV injection. (C) Proposed pathways by which TF+ TMVs enhance venous thromboembolism (VTE).

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