Figure 2. Chronic hypoxia induces early RV angiogenesis.

In (A), representative images of RV isotropic uniform random (IUR) sections stained with markers specific for endothelial cell glycoproteins (isolectin IB₄) and myocyte plasma membrane glycoproteins (wheat germ agglutinin; WGA) are shown. IB4 stains endothelial cells of capillaries and larger resistance vessels, though only the former were quantified. Bar = 10 µm. Images were used for stereological assessment in animals exposed to normoxia (Norm) and chronic hypoxia (CH) for 7 days. In (B), RV volume and RV CM volume increase with CH. As shown in (C), RV capillary length, surface area, and luminal volume are significantly increased after 7 days of CH. P-values are for Student’s t-test.