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a) After PCR, 519 bp amplicons are produced in the drops containing SV40 virions. These amplicons incorporate dUTP and are digested after USER treatment. b, c) Restriction analysis is used to verify successful amplification of SV40 genome. b) After PvuII treatment, the genomic copies of SV40 are cleaved into three fragments of 1446, 1790 and 1997 bp. c) At least 100 PCR positive drops are required to obtain a detectable amount of the SV40 genome sequence by MDA.
