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. Author manuscript; available in PMC: 2017 Jan 12.
Published in final edited form as: Cell Metab. 2015 Nov 19;23(1):113–127. doi: 10.1016/j.cmet.2015.10.008

Fig. 4. Blocking POMCergic autophagy inhibits lipophagy.

Fig. 4

(A) Plan to analyze autophagy in BAT/liver. (B) Immunoblot (IB) for LC3-II in BAT, and (C) liver explants from RT-housed and 1 hr cold-exposed control and Atg7KOPOMC mice treated or not with Lys Inh for 2 hr, n=3. (D) IB for indicated proteins in BAT LD, and (E) liver LD from RT-housed and 1 hr cold-exposed control and Atg7KOPOMC mice (5 livers and 5 BAT were pooled per sample per experiment, n=2). (F) BODIPY 493/503 staining in BAT from RT-housed and 1 hr cold-exposed control and Atg7KOPOMC mice, n=3. (G) Oil Red O staining in liver from 1 hr cold-exposed control and Atg7KOPOMC mice, n=3. (H–I) BAT and (J–K) liver oxygen consumption rates (OCR) and AUC (area under curve) from 5–6 mo RT-housed and 1 hr cold-exposed Atg7KOPOMC male mice, n=4. (L) OCR in BAT from 1 hr cold-exposed male control and Atg7KOAgRP male mice, n=3. (M) Rectal temperature in control and Atg7KOPOMC male mice at RT and after 15 min and 30 min of cold exposure, n=5. Bars are mean±s.e.m. *P<0.05, **P<0.01; Student’s t-test. Ponceau is loading control. See also Figure S4.