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. Author manuscript; available in PMC: 2017 Jan 12.
Published in final edited form as: Cell Metab. 2015 Nov 19;23(1):113–127. doi: 10.1016/j.cmet.2015.10.008

Fig. 6. Lipophagy failure in denervated BAT.

Fig. 6

(A) One BAT pad in each mouse was denervated (DNV), and DNV and contralateral intact BAT pad were analyzed for autophagy. (B) MBH cannulation, and intra-MBH DMSO or Rap injections for 2 hr and autophagy analyses in intact and DNV BAT from intra-MBH DMSO or Rap-injected mice. (C) Immunoblot (IB) for LC3, and quantification for LC3-II/LC3-I ratios, and net LC3-II flux in intact and DNV BAT from intra-MBH DMSO or Rap-injected 5–6 mo male mice, n=4. (D) qPCR for indicated genes in intact and DNV BAT from intra-MBH Rap-injected 5–6 mo male mice, n=3–5. (E) IB for LC3 and quantification for LC3-II/LC3-I ratios in LD from intact and DNV BAT from intra-MBH DMSO or Rap-injected 5-6 mo male mice (5 BAT pads pooled per sample per experiment, n=2). (F) BODIPY staining in intact and DNV BAT from intra-MBH DMSO or Rap-injected 5–6 mo mice, n=3. (G) Staining for BODIPY and LC3 in BAT from intra-MBH DMSO or Rap-injected 5–6 mo male Con and Atg7KOPOMC mice, n=3. Bars are mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001; Student’s t-test. Ponceau is loading control.