Table 1.
Primary antibody | Corresponding secondary antibody |
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---|---|---|---|---|---|---|
Antiserum | Host | Antigen (Immunogen) |
Description (Specification) |
Code & Vendor (Manufactu rer) |
Method & Dilution |
Name, method, dilution |
Anti-GAT1 | Rabbit | Peptide (C)ERNMHQ MTDGLDK, corresponding to amino acids residues 194-206 of rat GAT1 (Accession P23978). 2nd extracellular loop. |
Extracellular domain, affinity purified polyclonal antibody, SLC6A1; supplied with control antigen to GAT1 (blocking peptide) |
AGT-001 Alomone2 |
WB; 1:500; 1:1000 with blocking peptide |
WB; 1:5000, donkey polyclonal anti-rabbit IgG-HRP, sc-2313, Santa Cruz9 |
Anti-GAT1 | Rabbit | C-terminus of rat GAT1 (aa 588-599) |
Affinity purified polyclonal antibody |
AB1570 Millipore5 |
IHC; 1:100 | IHC; 1:100, donkey-anti- rabbit IgG DyLight 549 (#711-505-152), Jackson4 |
Anti-GAT1 | Rabbit | Peptide corresponding to amino acid residues from the C-terminal region of rat GAT1 |
Affinity purified polyclonal IgG antibody, detects a band of approximately 67 kDa; SLC6A1 |
ab72448 Abcam1 |
IHC; 1:500 EM; 1:50 |
IHC; 1:500, donkey anti- rabbit IgG DyLight 594 preadsorbed, ab96921, Abcam1 |
Anti-GAT1 | Rabbit | 20 residue C- terminal synthetic peptide (Rat) |
polyclonal antibody, reacts specifically with a 72 kDa protein; SLC6A1 |
ab426 Abcam1 |
IHC; 1:1000 | IHC; 1:500, donkey anti- rabbit IgG DyLight 594 preadsorbed, ab96921, Abcam1 |
Anti-GAT3 | Rabbit | C-terminus of rat GAT3 (aa 607-627) coupled to KLH |
Affinity purified polyclonal antibody |
AB1574 Millipore5 |
WB; 1:1000 IHC; 1:100, 1:500 EM; 1:50 |
WB; 1:4000, IRDye 680 Goat Anti-Rabbit IgG (#926-32221),LI-COR6 IHC; 1:100, donkey-anti- rabbit IgG DyLight 488 (#711-485-152), Jackson4 IHC; 1:500, donkey anti- rabbit IgG DyLight 594 preadsorbed, ab96921, Abcam1 |
Anti-GAT3 | Mouse | C-terminus of rat GAT3 (aa 601-625) |
Monoclonal [B-6] IgG2b |
sc-376001, Santa Cruz9 |
IHC; 1:1000 | IHC; 1:500, Donkey Anti- Mouse IgG Alexa Fluor 488, (#715-546-151), Jackson4 |
Anti- GAPDH |
Mouse | Glyceraldehyd e-3-phosphate dehydrogenase from rabbit muscle |
Purified monoclonal antibody, clone 6C5, recognizes a 36 kDa band of the reduced monomer |
MAB374 Millipore5 |
WB; 1:8000 | WB; 1:200000, Rabbit polyclonal anti-mouse IgG-HRP, AP160P, Millipore3 WB; 1:3000, IRDye 800CW Goat Anti-Mouse IgG (#926-32210), LI- COR6 |
Anti-GFAP | Mouse | Purified glial filament (Debus, E., 1983) |
Purified monoclonal antibody, clone GA5 |
MAB3402 Millipore5 |
IHC (slices); 1:500 IHC (cell culture); 1:500 |
IHC; 1:500, Donkey Anti- Mouse IgG Alexa Fluor 488, (#715-546-151), Jackson4 |
Anti-NFH | Hen | Purified IgY fractions from eggs |
Purified neurofilament protein (200 kDa) |
NFH AVES3 |
IHC; 1:500 | IHC; 1:500, Donkey Anti- Chicken IgY CF488A, SAB4600031-50, Sigma- Aldrich10 |
Anti-NeuN | Mouse | Purified cell nuclei from mouse brain |
Purified monoclonal antibody, clone A60, IgG conjugated to Alexa Fluor®488 |
MAB377X Millipore5 |
IHC; 1:500 | |
Anti-NSE | Rabbit | Synthetic peptide derived from human NSE |
Affinity purified polyclonal to NSE antibody |
ab53025 Abcam1 |
IHC; 1:500 | IHC; 1:500, Donkey Anti- Rabbit IgG DyLight 594 preadsorbed, ab96921, Abcam1 |
Anti-AVP | Guinea pig |
Synthetic peptide as the immunogen, epitope has not been mapped |
Anti-(Arg8)- Vasopressin, undiluted, lyophilized polyclonal not purified antiserum, |
S-3100 Peninsula8 |
IHC; 1:2000 | IHC; 1:500, Goat Anti- Guinea Pig IgG Alexa Fluor 594, (A-11076), Life Technologies7 |
Anti-VIP | Rabbit | Full length protein VIP (Pig) conjugated to Bovine thyroglobulin by a CDI linker |
Whole antiserum polyclonal IgG |
ab43841 Abcam1 |
IHC; 1:250 | IHC; 1:500, Donkey Anti- Rabbit IgG DyLight 594 preadsorbed, ab96921, Abcam1 |
Abcam Inc., Cambridge, MA
Alomone Labs Ltd., Jerusalem, Izrael
Aves Labs, Tigard, OR
Jackson ImmunoResearch Laboratories, Inc., West Grove, PA
Millipore (Chemicon), Temecula, CA
LI-COR Biosciences, Lincoln, NE
Life Technologies, Grand Island, NY
Peninsula Laboratories International Inc., San Carlos, CA
Santa Cruz Biotechnology Inc., CA
Sigma-Aldrich, Saint Louis, MO