Table 1.

Primary and secondary antibodies used in the present studies.

Primary antibody						Corresponding secondary antibody
Antiserum	Host	Antigen (Immunogen)	Description (Specification)	Code & Vendor (Manufactu rer)	Method & Dilution	Name, method, dilution
Anti-GAT1	Rabbit	Peptide (C)ERNMHQ MTDGLDK, corresponding to amino acids residues 194-206 of rat GAT1 (Accession P23978). 2nd extracellular loop.	Extracellular domain, affinity purified polyclonal antibody, SLC6A1; supplied with control antigen to GAT1 (blocking peptide)	AGT-001 Alomone2	WB; 1:500; 1:1000 with blocking peptide	WB; 1:5000, donkey polyclonal anti-rabbit IgG-HRP, sc-2313, Santa Cruz9
Anti-GAT1	Rabbit	C-terminus of rat GAT1 (aa 588-599)	Affinity purified polyclonal antibody	AB1570 Millipore5	IHC; 1:100	IHC; 1:100, donkey-anti- rabbit IgG DyLight 549 (#711-505-152), Jackson4
Anti-GAT1	Rabbit	Peptide corresponding to amino acid residues from the C-terminal region of rat GAT1	Affinity purified polyclonal IgG antibody, detects a band of approximately 67 kDa; SLC6A1	ab72448 Abcam1	IHC; 1:500 EM; 1:50	IHC; 1:500, donkey anti- rabbit IgG DyLight 594 preadsorbed, ab96921, Abcam1
Anti-GAT1	Rabbit	20 residue C- terminal synthetic peptide (Rat)	polyclonal antibody, reacts specifically with a 72 kDa protein; SLC6A1	ab426 Abcam1	IHC; 1:1000	IHC; 1:500, donkey anti- rabbit IgG DyLight 594 preadsorbed, ab96921, Abcam1
Anti-GAT3	Rabbit	C-terminus of rat GAT3 (aa 607-627) coupled to KLH	Affinity purified polyclonal antibody	AB1574 Millipore5	WB; 1:1000 IHC; 1:100, 1:500 EM; 1:50	WB; 1:4000, IRDye 680 Goat Anti-Rabbit IgG (#926-32221),LI-COR6 IHC; 1:100, donkey-anti- rabbit IgG DyLight 488 (#711-485-152), Jackson4 IHC; 1:500, donkey anti- rabbit IgG DyLight 594 preadsorbed, ab96921, Abcam1
Anti-GAT3	Mouse	C-terminus of rat GAT3 (aa 601-625)	Monoclonal [B-6] IgG2b	sc-376001, Santa Cruz9	IHC; 1:1000	IHC; 1:500, Donkey Anti- Mouse IgG Alexa Fluor 488, (#715-546-151), Jackson4
Anti- GAPDH	Mouse	Glyceraldehyd e-3-phosphate dehydrogenase from rabbit muscle	Purified monoclonal antibody, clone 6C5, recognizes a 36 kDa band of the reduced monomer	MAB374 Millipore5	WB; 1:8000	WB; 1:200000, Rabbit polyclonal anti-mouse IgG-HRP, AP160P, Millipore3 WB; 1:3000, IRDye 800CW Goat Anti-Mouse IgG (#926-32210), LI- COR6
Anti-GFAP	Mouse	Purified glial filament (Debus, E., 1983)	Purified monoclonal antibody, clone GA5	MAB3402 Millipore5	IHC (slices); 1:500 IHC (cell culture); 1:500	IHC; 1:500, Donkey Anti- Mouse IgG Alexa Fluor 488, (#715-546-151), Jackson4
Anti-NFH	Hen	Purified IgY fractions from eggs	Purified neurofilament protein (200 kDa)	NFH AVES3	IHC; 1:500	IHC; 1:500, Donkey Anti- Chicken IgY CF488A, SAB4600031-50, Sigma- Aldrich10
Anti-NeuN	Mouse	Purified cell nuclei from mouse brain	Purified monoclonal antibody, clone A60, IgG conjugated to Alexa Fluor®488	MAB377X Millipore5	IHC; 1:500
Anti-NSE	Rabbit	Synthetic peptide derived from human NSE	Affinity purified polyclonal to NSE antibody	ab53025 Abcam1	IHC; 1:500	IHC; 1:500, Donkey Anti- Rabbit IgG DyLight 594 preadsorbed, ab96921, Abcam1
Anti-AVP	Guinea pig	Synthetic peptide as the immunogen, epitope has not been mapped	Anti-(Arg8)- Vasopressin, undiluted, lyophilized polyclonal not purified antiserum,	S-3100 Peninsula8	IHC; 1:2000	IHC; 1:500, Goat Anti- Guinea Pig IgG Alexa Fluor 594, (A-11076), Life Technologies7
Anti-VIP	Rabbit	Full length protein VIP (Pig) conjugated to Bovine thyroglobulin by a CDI linker	Whole antiserum polyclonal IgG	ab43841 Abcam1	IHC; 1:250	IHC; 1:500, Donkey Anti- Rabbit IgG DyLight 594 preadsorbed, ab96921, Abcam1

¹Abcam Inc., Cambridge, MA

²Alomone Labs Ltd., Jerusalem, Izrael

³Aves Labs, Tigard, OR

⁴Jackson ImmunoResearch Laboratories, Inc., West Grove, PA

⁵Millipore (Chemicon), Temecula, CA

⁶LI-COR Biosciences, Lincoln, NE

⁷Life Technologies, Grand Island, NY

⁸Peninsula Laboratories International Inc., San Carlos, CA

⁹Santa Cruz Biotechnology Inc., CA

¹⁰Sigma-Aldrich, Saint Louis, MO