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Performance characteristics of the 5-hydroxymethycytosine (5-hmC) immunohistochemical assay. A receiver operating characteristic (ROC) curve was generated for 5-hmC immunohistochemical staining score for distinguishing nevi from malignant melanoma, using the combined cohorts. The cutoff was set at an immunohistochemical staining score = 17 to achieve optimal sensitivity and specificity. AUC, area under the curve.
