Effects of NBT treatment on protein levels (A) and mRNA levels (B) of IL-1β, IL-6, and TNF-α in the colonic mucosa of AOM/DSS-treated mice. Colon tissues samples were randomly collected from the middle and distal colon, and then subjected to ELISA or qRT-PCR analysis. Data are shown as the mean ± SD of three independent experiments. The amount of IL-1β, IL-6, TNF-α mRNA expression was normalized to that of β-actin. * indicates statistically significant differences from the control group (p < 0.01, n = 3).