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. Author manuscript; available in PMC: 2016 Dec 8.
Published in final edited form as: Biofactors. 2015 Dec 8;41(6):391–402. doi: 10.1002/biof.1240

Figure 4. Effect of leucine restriction on remodeling of inguinal WAT.

Figure 4

Mice were fed control (CON) or leucine-restricted (LR) diets for 11 weeks prior to harvest of tissue for analysis of (A) mRNA levels of browning genes, (B) western blot of UCP1 protein in IWAT, and (C) representative IHC images of IWAT from mice fed the CON or LR diets. Scale bar: 400 μm. Cell membranes were stained with wheat germ agglutinin (green) and lipid droplet membranes were stained with perilipin (red) to detect multilocular cells. (D) Number of multilocular, beige adipocytes graphed as percent of total adipocytes in each dietary group. Means ± SEM are presented for each variable and based on n=9 animals per group for A, n=6 per group for B, and n=4 per group for C&D. Means annotated with asterisks differ at P<0.05.