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Quantification and characterization of Class II MHC-eGFP+ cells from 30 μL of whole blood. a, Fluorescence microscopy of cells captured on VHH7-modified and unmodified GO substrates. b, Quantification of captured cells. c, Confocal microscopy of captured cells. d, Higher magnification of a captured cell, showing colocalization of the Class II MHC-eGFP signal with VHH7-TAMRA. e, Confocal microscopy images of VHH7-captured cells after staining with Alexa647-labeled VHH7.
