Figure 5.

Quantification and characterization of captured CD11b+ cells by combined VHH7-VHH DC13-modified substrates. a, Cells were first passed over a VHH7 coated surface and captured subsequently on VHH DC13-modified substrates. b, Characterization of captured cells. c, Fraction of captured cells. d, Comparison of the yield of CD11b+/eGFP− cells captured on a single (VHH DC13 alone) or dually modified VHH7-VHH DC13 substrate. e, Confocal microscopy of captured cells engaged in phagocytosis of Candida albicans. Captured cells were stained with Alexa647-labeled anti-CD11b antibody while Candida albicans expressed blue fluorescent protein to facilitate their detection.