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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: J Neurochem. 2015 Oct 8;135(5):987–997. doi: 10.1111/jnc.13352

Figure 1.

Figure 1

Sites for Acrolein Injection and Mechanical Allodynia Von Frey Test Left. Injection of acrolein directly to T10 of rat spinal cord. 40 nmol acrolein was injected into the right side of the cord while saline was injected into the left side as a control. Right. For the acrolein injection into the paw, a 30 gauge needle was inserted at the site of A (heel of the rat’s hindlimb foot), and advanced to site B, where an acrolein solution (25 μl, 625nmol) was injected intradermally. Acrolein-induced mechanical hypersensitivity was assessed by applying Von Frey filaments at site C.