Figure 7.

Stability of transgene expression over time. In-gel fluorescence analyses of YFP expression in the different transformants of strains UVM11 and Elow47 are shown. Samples of 12 μg total soluble protein were separated by non-denaturing SDS-PAGE. Protein samples extracted from non-transformed UVM11 or Elow47 were used as negative control (NC). A dilution series (0.6, 1.2, 3, 6 and 12 μg TSP) of a YFP-expressing strain (CrYFP transformant number 2 in UVM11) was loaded onto each gel as a positive control (PC) and a standard for comparisons between gels. Gels were scanned using a Typhoon scanner and applying the 526 SP Fluorescein filter. L: protein ladder (molecular weight marker); ---: empty lane in the gel.

(a) YFP accumulation in transformants generated with CrCpYFP, YFPla, vYFP and CrYFP in strains UVM11 and Elow47 one month after transformation.

(b) YFP accumulation in the same transformants four months after transformation. The transformed strains were maintained under non-selective conditions. See also Figure 3 which shows the same fluorescence scans and, additionally, loading controls for all gels.