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. 2016 Jan 16;16:25. doi: 10.1186/s12885-015-2029-8

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© Kampa-Schittenhelm et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Reduction of the viable leukemia cohort upon treatment with THC. a FSC/SSC scatter plot was used to gate (R1) the viable cell population. Counted cells (total) n = 30,000. b Viability of the R1-gated population was confirmed in an annexin V/PI-based apoptosis assay (viable population located in the lower left (LL) section of a quadrant plot). c Immunophenotyping assay to distinguish the CD34 (PE conjugated) and/or CD45low (FITC conjugated) positive leukemia population is shown. This cohort was followed prior to and 48 h after exposure to THC to determine reduction of viable cells in response to THC (d and e). A representative patient sample is shown. Percental waterfall plots are provided for AML (f) and ALL (g) for all tested patient samples