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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Mol Microbiol. 2015 May 9;97(1):166–178. doi: 10.1111/mmi.13018

Fig 3.

Fig 3

EptAPa adds pEtN exclusively to the lipid A 4' phosphate group. A) Cells were grown in LB broth. Major 32P-labeled lipid A species are indicated with a cartoon corresponding to the lipid A structure; colors of modification groups are the same as those used in Fig. 1. Heterologous expression of eptAPa in BN2 results in a pEtN-modified species, while expression of either the lpxEFn or lpxFFn phosphatase results in an increased of monophosphorylated species. Co-expression of lpxEFn and eptAPa results in pEtN addition to the 1-dephosphorylated lipid A molecule, while no pEtN addition of 4' –dephosphorylated species is detected. B) MALDI-TOF analysis of lipid A isolated from BN2 coexpressing lpxEFn and eptAPa corroborates the presence of a monophosphorylated, pEtN-modified species. C) MALDI-TOF analysis of lipid A isolated from BN2 coexpressing lpxFFn and eptAPa reveals that when the 4' phosphate group is removed, pEtN addition does not occur.