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. Author manuscript; available in PMC: 2016 Jan 17.
Published in final edited form as: Mol Microbiol. 2013 Jan 21;87(4):713–729. doi: 10.1111/mmi.12089

Figure 1. The TbLOK1 protein is essential for trypanosome growth.

Figure 1

A. Knockdown of TbLOK1 stops cell division. Cells were grown without (−RNAi) or with (+RNAi) induction of TbLOK1 RNAi, and then parasites were counted. Values on the Y-axis are the measured cell number times the dilution factor. B. TbLOK1 mRNA levels decrease following RNAi induction. At the indicated times, total RNA was electrophoresed, blotted and probed with TbLOK1 or the tubulin gene (load control). RNA from equivalent number of cells was loaded in each lane. C. The TbLOK1 protein levels drop following RNAi. At the indicated times, cells were isolated and extracted proteins were electrophoresed and western blotted with antibodies to TbLOK1, TbHSP70mt and enolase (a load control). Proteins from equivalent numbers of cells were loaded in each lane.