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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Proteomics. 2015 Nov 6;16(2):241–256. doi: 10.1002/pmic.201500266

Figure 7. Systematic approaches for analysis of protein N-glycosylation.

Figure 7

Glycans, deglycopeptides and intact glycopeptides are systematically analyzed using (A) In-solution and (B) GIG. Intact glycopeptides are searched using databases from N-glycans and global peptides. In solution, peptides (A.1) are quantitatively analyzed by LC-MS/MS while N-glycans can be determined by labeling or permethylation (A.2); intact glycopeptides are enriched by chromatography (HILIC) or lectin (A.3). On GIG, glycoproteins are immobilized on resin for derivatization of glycan; sialic acids are stabilized (B.2) before tryptic digestion for glycopeptide identification (B3); deglycosylated peptides are quantitatively analyzed by LC-MS/MS (B.1).