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. Author manuscript; available in PMC: 2017 Jan 15.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Dec 8;469(3):449–455. doi: 10.1016/j.bbrc.2015.11.134

Fig. 2.

Fig. 2

Rad6 expression correlates with stemness and platinum resistance in OC cells. A. Isogenic platinum-sensitive (A2780) platinum-resistant (A2780/CP70) ovarian cancer cells were tested for capacity for anchorage-independent growth (as described in Methods & Materials). B. The large (>50 cells) and small (<50 cells) OC spheres formed by each cell line were counted and average value presented. C. Anchorage-independent growth (represented by OC sphere formation) is characteristic of CSCs; therefore, protein was harvested from these cells and analyzed for the Rad6 levels, the presence of stem cell markers (ALDH1A1 & BMI1), expression of signaling molecules that promote stemness (Gli1 & β-catenin), and ubiquitination of histone 2B by Rad6 and related downstream histone H3 trimethylation H3K79me3. D. The isogenic A2780 and A2780/CP70 cell lines were analyzed by Matrigel invasion assay to determine invasive potential of these cells. Statistical analysis: * indicates P<0.05, **P<0.01.