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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Microbes Infect. 2015 Sep 8;18(1):21–29. doi: 10.1016/j.micinf.2015.08.015

Fig. 3.

Fig. 3

YopJ and the T3SS are required for Y. pestis inhibition of IL-8 secretion by PMNs. IL-8 secretion by human PMNs incubated with Y. pestis lacking yopE, -H, -T, -J, -M or ypKA was compared to PMNs incubated with the parental KIM5 or with KIM6+ (pCD1Δ1234). In addition, IL-8 secretion by PMNs incubated with the KIM5ΔyopJ or KIM6 strains that had been transformed with the low- or high-copy number complementation plasmids (pWKS::yopJ and PCR::yopJ, respectively) was determined. Culture supernatant was collected after 12 h (A) or 24 h (B) of incubation and the concentration of IL-8 was determined by ELISA. The mean and SEM for at least three independent experiments are indicated. Data were analyzed by one-way ANOVA with Tukey post-test. *, P < 0.05 compared to KIM5.