Table 2.
Human Serum Samples (1/10 dilution)* | |||
---|---|---|---|
Spiked concentrations (pg/mL) | Measured concentrations (pg/mL) | Recovery (%) | RSD (%) |
13 | 5.31 ± 3.66 | 40.8 | 28.2 |
30 | 15.58 ± 4.83 | 51.9 | 16.1 |
71.8 | 67.57 ± 6.95 | 94.1 | 9.7 |
100 | 94.90 ± 17.18 | 94.9 | 17.2 |
The non-spike serum sample was used as reference.
The parameters were extracted from the four-parameter equation used to fit the standard curve. The measured concentrations were obtained after subtract the native MCP-1 concentration in the serum samples (5.6 pg/mL, 1/10 dilution).