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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Mol Cancer Res. 2015 Sep 22;14(1):35–43. doi: 10.1158/1541-7786.MCR-15-0295

Figure 2. HDACi induces G2 cell cycle arrest and apoptosis in ES cells in vitro.

Figure 2

(A) Propidium iodide-FACS analysis abexinostat (0.5, 1 μM/48 h) induced a significant increase in the G2/M phase (48 h) in tested ES cells (P < 0.05). VAESBJ exhibited the strongest G2/M phase shift after abexinostat treatment compared to HS-ES and Epi-544. (B) Annexin V/PI-FACS analysis demonstrated a significant increase in abexinostat-induced (1 μM/48 h) apoptosis was observed in all three ES cell lines. (C) Western blot analysis confirmed Annexin V data where abexinostat (1 μM/48 h) induced an increase in apoptosis markers cleaved PARP and cleaved caspase 3.