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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Exp Neurol. 2015 Sep 12;276:72–82. doi: 10.1016/j.expneurol.2015.09.004

Figure 6. mRNA expression in propriospinal neurons.

Figure 6

(A) T13 spinal cord grey matter (dotted line) showing regenerating neurons labeled with TB placed at distal end of a PNG and stained for GAP43 mRNA (scale bar = 200μm). The inset shows TB positive neurons. Dash lined box is the region of interest for assessing propriospinal neurons in intermediate gray. a1 shows a higher magnification image of TB positive neuron stained for GAP43 mRNA (a2) and a3 shows a merged image. Similarly, b1-b3 and c1-c3 show individual TB positive neurons stained for βactin and Neuritin mRNAs respectively (scale bar = 10μm). (B, C, D) Y-axis shows average of FISH signal intensity per neuron for GAP43, β-actin, Neuritin mRNAs respectively. X-axis shows the animal groups. * indicates significant difference (p<0.05). + indicates significant difference (p<0.05) from uninjured control.