Fig 1. Schedule for the biologic replicates, diet, sporozoite infection and liver harvest.

In replicate 1 mice were purchased at 5 months of age and split into two groups based on phlebotomy (nonphlebotomized groups are not shown on the shcme; they followed the same pattern of diet change after anemia confirmation on phlebotomized mice) with generation of four groups based on two different diets which were then sampled after 2 and 6 weeks with a change in diet. For the second replicate 3 week old mice were maintained on a low iron diet which did not change hemoglobin or ZnPPIX and after 5 months the mice on the low iron diet were phlebotomized and split into groups as in replicate 1.