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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Mol Cancer Res. 2015 Sep 18;14(1):66–77. doi: 10.1158/1541-7786.MCR-15-0159

Figure 6. Levels of TSC2 and Sestrin2 mRNA and protein in carcinoma cells that are p53 null or that express only mutant p53.

Figure 6

(A) TSC2 protein levels determined by immunoblot of lysates. (B) TSC2 and Sestrin2 mRNA levels were measured by RT-qPCR using total RNA. (mean ± sd, n=3). siRNA depletion of TSC2 (C) or genetic deletion of Sestrin2 (D) substantially increase the level of mTORC1 activity. HCT116 cells wt for p53 were transfected with siRNA against TSC2 or a scrambled control and mTORC1 activity was assessed by phosphorylation of S6K1 at T389 (C). MEFs from mice with genetic deletion of Sestrin2 were likewise probed for pT389-S6K1 (D).

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