Homozygous db mice showed an increase in tau phosphorylation, as determined by 96-well slot blot (shown: phospo-tau [AT8]; total tau [HT7] and β-actin [AC15]; p < 0.02, n = 22 Lepr+/+, 50 Lepr+/db, 24 Leprdb/db). The increase was also significant when not standardized to total tau (p < 0.03, not shown). No difference between sexes was observed (p = 0.182, n = 45 female, 51 male).