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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Am J Reprod Immunol. 2015 Dec 15;75(2):134–145. doi: 10.1111/aji.12458

Figure 1. Representative flow cytometry gating strategy for enumeration of gamma delta (GD) T cell subpopulation in endocervical mucosa.

Figure 1

Identification of endocervical T cell populations by following gating strategy: A) Large gate is set on endocervical cytobrush cells B) Exclusion of cell doublets C) Live cells are viability dye-negative population D) CD45 expression on live cells E) CD3 expression on CD45+ cells F) CD4 and CD8 expression on CD3+ cells G) TCRVD1 (GD1) and TCRVD2 (GD2) expression on CD3+ cells H) CD4 and CD8 expression on GD1+ cells I) TCRVD1 expression on gated CD3 negative cells.