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. Author manuscript; available in PMC: 2017 Jan 15.
Published in final edited form as: Clin Cancer Res. 2015 Aug 31;22(2):436–447. doi: 10.1158/1078-0432.CCR-15-1070

Figure 3. Expression of Inhibitory Receptors (IRs) on human T cells.

Figure 3

TILs were isolated as described in Figure 2 and subjected to FACS using anti-human CD45, CD4, TCRVβ13.1, PD1, Tim3, Lag3 antibodies.

A) Expression of IRs was examined on the CD4 positive and negative (CD8) cells. The number in the box represents the percentage of IR+ T cells in the CD4 or CD8 subset.

B) Upregulation of IR expression was similar between CD8 and CD4 TILs.

C) Expression of IRs was examined on the TCR+ positive (TCRVβ13.1+) and negative cells. The number in the box represents the percentage of IR+ T cells in the TCRVB13.1+ or TCRVB13.1− subset.

D) Compared to TCR− TILs, TCR+ TILs demonstrated significantly greater upregulation of PD1 (74% vs. 36%, p<0.01) and Tim3 (49% vs. 41%, p<0.05).

Dot-plots display representative analyses from individual single mouse. Bar graph displays average frequency of IR+ events in TCR+ and TCR− gates ± S.E. from individual mice.