Sterilization |
Critical instruments |
High-temperature sterilization |
Steam |
|
Dry heat |
|
Low-temperature sterilization |
Ethylene oxide gas |
|
Hydrogen peroxide gas plasma |
|
Chemical sterilization (immersion) |
≥2% Glutaraldehyde |
20-25℃, 10 h |
7.5% Hydrogen peroxide |
6 h |
0.2% peracetic acid |
50 min |
7.35% Hydrogen peroxide + 0.23% peracetic acid |
3 h |
1.0% hydrogen peroxide + 0.08% peracetic acid) |
8 h |
High-level disinfection |
Semi-critical instruments |
Chemical sterilization (immersion) |
≥2% Glutaraldehyde |
2%: 20℃, 20 min |
2.5%: 35℃, 5 min |
0.55% ortho-phthalaldehyde |
20℃, 12 min |
25℃, 5 min |
7.5% hydrogen peroxide |
30 min |
7.35% hydrogen peroxide + 0.23% peracetic acid |
15 min |
1.0% hydrogen peroxide + 0.08% peracetic acid |
25 min |
650-675 ppm hypochlorite (prepared in situ through electrolysis) |
10 min |
Intermediate-level disinfection |
Partially semi-critical instruments, non-critical instruments |
(contact duration ≥1 min) |
Active chlorine ≥1,000 ppm |
|
Sodium hypochlorite |
Phenolic disinfectant |
Iodophor disinfectant |
70-90% alcohol formulation (dthanol/isopropanol) |
Low-level disinfection |
Non-critical instruments |
Chemical sterilization (immersion; contact duration ≥1 min) |
Active chlorine ≥100 ppm |
|
Sodium hypochlorite |
Phenolic disinfectants |
Iodophor disinfectants |
Quaternary ammonium salt formulation |
70-90% alcohol formulation (ethanol/isopropanol) |