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. 2015 Dec 30;47(4):278–302. doi: 10.3947/ic.2015.47.4.278

Table 12. Application scope and disinfection method depending on sterilization and disinfection levels.

Category Scope Method Required time
Sterilization Critical instruments High-temperature sterilization Steam
Dry heat
Low-temperature sterilization Ethylene oxide gas
Hydrogen peroxide gas plasma
Chemical sterilization (immersion) ≥2% Glutaraldehyde 20-25℃, 10 h
7.5% Hydrogen peroxide 6 h
0.2% peracetic acid 50 min
7.35% Hydrogen peroxide + 0.23% peracetic acid 3 h
1.0% hydrogen peroxide + 0.08% peracetic acid) 8 h
High-level disinfection Semi-critical instruments Chemical sterilization (immersion) ≥2% Glutaraldehyde 2%: 20℃, 20 min
2.5%: 35℃, 5 min
0.55% ortho-phthalaldehyde 20℃, 12 min
25℃, 5 min
7.5% hydrogen peroxide 30 min
7.35% hydrogen peroxide + 0.23% peracetic acid 15 min
1.0% hydrogen peroxide + 0.08% peracetic acid 25 min
650-675 ppm hypochlorite (prepared in situ through electrolysis) 10 min
Intermediate-level disinfection Partially semi-critical instruments, non-critical instruments (contact duration ≥1 min) Active chlorine ≥1,000 ppm
Sodium hypochlorite
Phenolic disinfectant
Iodophor disinfectant
70-90% alcohol formulation (dthanol/isopropanol)
Low-level disinfection Non-critical instruments Chemical sterilization (immersion; contact duration ≥1 min) Active chlorine ≥100 ppm
Sodium hypochlorite
Phenolic disinfectants
Iodophor disinfectants
Quaternary ammonium salt formulation
70-90% alcohol formulation (ethanol/isopropanol)