Figure 1.

The MRN complex is transcriptionally controlled by MYCN. (a) Western blot (WB) analysis of MYCN and MRN protein components in the LAN5 and SK-N-BE neuron-like cell models. (b, c) Analysis of the expression of the indicated proteins (b) and transcripts (c) in the SHEP Tet 21/N inducible-MYCN overexpression model. Transcripts expression were normalized on GAPDH levels and given as fold induction compared with MYCN uninduced controls. (d) Organization of the promoters of the MRE11, RAD50 and NBS1 human genes. Typical E-Boxes (CACGTG) are present in the human MRE11, RAD50 and NBS1 promoters at the indicated positions. Black arrows indicate exons one and two, and nucleotide positions are given from the first nucleotide of exon 1, for each gene. P1, P2 and P3 indicate GC-reach sequences of the NBS1 promoter. (e) PCR amplification of immunoprecipitated chromatin prepared from MYCN induced or uninduced SHEP Tet 21/N (MYCN+/−) cells. Chromatin was incubated with anti-IgG (IgG), anti-acetyl histone 3 (acH3) or with anti-MYCN antibodies. Prothymosin alpha (Ptmα) and β-actin primers were used as positive and negative controls, respectively