Figure 5.

Mycn promotes MRN complex expression in Hh-induced mouse cerebellar GCPs. (a and b) WB and real-time Q-PCR analysis of the expression of the indicated proteins/transcripts in mouse cerebella at the indicated post-natal (P) days; Zic1 and gamma-aminobutyric acid receptor subunit alpha-6 (Gabra6) were used as differentiation markers and the ponceau staining was used as the loading control. For Q-PCR, the levels of mRNAs were normalized to Hprt expression and reported as fold variation compared with P5 levels. (c) β-tubulin immunofluorescent staining and WB analysis of Mycn and MRN complex proteins in GPC cultures (DIV: days in vitro). (d) Q-PCR assessment of the expression of the indicated transcripts normalized as above and expressed as fold variation compared with the levels at DIV 1. (e–g) WB (e and g) and real-time Q-PCR analysis (f) of the expression of the indicated proteins/transcripts in GPCs treated with DMSO or SAG for 48 h without (e–g) or with (g) lentiviral-transduced anti-Mycn shRNAi