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. 2015 Jun 12;23(2):197–206. doi: 10.1038/cdd.2015.81

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The MRN complex controls replication stress during HH-induced mouse cerebellar GCPs proliferation. (a) WB analysis of Mycn protein levels in freshly isolated GPC (DIV0), SAG treated (48 h) GPCs and MYCN+ cells. (b) Tunel assays in GPCs cultures pre-treated with SAG and then incubated with mirin for 18 h. (c) Quantitation of the cells showing more than three 53BP1 foci in GPCs cultures pre-treated with SAG and then incubated with mirin (6 h). (d) WB analysis of the expression of the indicated proteins and phosphoepitopes in cells treated as in panel c. (*P<0.05)