Figure 2.

Klf4 ablation altered the differentiation pattern in mouse lung. (a) Genetic model of the Rosa-Cre⁺;Klf4^fl/fl mouse. LoxP elements allow Cre-induced Klf4 deletion (top); and Rosa26 drives CreER^T2 expression in mouse lung (bottom). (b–d) Immunofluorescent staining for KLF4 (red) in wild-type mouse lung (b) and in Klf4-deleted mouse lung (c). (d) Statistical analysis of KLF4-positive cells in mouse lung. Data are represented as mean±S.D. (e–g) Immunofluorescent staining for SP-C in wild-type (e) and Klf4-deleted (f) mouse lung. (g) Statistical analysis of SP-C-positive cells in mouse lung. Data are represented as mean±S.D. Scale bars for (b and c): 30 μm. Scale bars for (e and f): 100 μm