Figure 1.

Genome-wide RNAi screen for inhibitors of erastin-induced ferroptosis. (a) siRNA targeting VDAC3 was used in HT-1080 fibrosarcoma cells. 10 nM siRNA was reverse transfected into cells in 384-well format. qPCR was used to verify knockdown in six-well plates. Error bars represent one S.D. using three biological replicates. (b) Screening format used to identify suppressors of erastin lethality. (c) Effect of 250 gene knockdowns on erastin lethality, ordered in rank of most significant in HT-1080 cells. On the right, gene knockdowns were filtered for lack of toxicity and t-test P-value<0.05, in both HT-1080 and Panc-1 cells