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Activity of 20 and CP55,940 in the [35S]GTPγS assay. Mean % changes in [35S]GTPγS binding to mouse brain membranes elicited by CP55,940 or 20. The mean EC50 value of CP55,940 with a 95% confidence interval is 27.3 nM (7.2–103.2 nM, n = 6), and its corresponding Emax value is 87.8% (67.9 and 107.7%). Vertical lines show SEM values.
