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. 2014 Nov 24;12(6):692–699. doi: 10.1038/cmi.2014.108

Figure 2.

Figure 2

RGC-32 expression during macrophage polarization was induced by IL-4 or LPS. (a) PMA-treated macrophages were polarized to M1 using a standard dose of LPS (100 ng/ml) in combination with graded doses of IFN-γ (0, 5, 10 and 20 ng/ml) for 42 h. (b) PMA-treated macrophages were stimulated using a standard dose of IFN-γ (20 ng/ml) in combination with graded doses of LPS (0, 25, 50 and 100 ng/ml) for 42 h. (c) PMA-treated macrophages were polarized to M2 using graded doses of IL-4 (0, 5, 10 and 20 ng/ml) for 42 h. PMA, phorbol 12-myristate 13-acetate; RGC-32, response gene to complement 32.