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. 2014 Nov 24;12(6):692–699. doi: 10.1038/cmi.2014.108

Figure 5.

Figure 5

Induction of RGC-32 by tumor-conditioned medium is dependent on M-CSF and IL-4. (a) RGC-32 mRNA expression in monocytes obtained from peripheral blood and TAMs from ascitic fluid was assayed by quantitative RT-PCR (n=12). (b) RGC-32 mRNA expression in monocytes exposed for 72 h to M-CSF, IL-4 and conditioned medium from the ascitic fluid of three colon carcinomas (CM1, CM2 and CM3), as determined by quantitative RT-PCR. (c) Inhibitory effect of anti–M-CSF and anti-IL-4 on RGC-32 mRNA levels induced by ascitic fluid obtained from metastatic colon carcinoma. The results are depicted as the RGC-32 mRNA levels detected in the presence of the anti-M-CSF and anti-IL-4 antibody relative to the levels observed in control cells treated with an isotype-matched control Ab. *P<0.05, **P<0.01. Ab, antibody; RGC-32, response gene to complement 32.