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. 2015 Feb 2;12(6):757–767. doi: 10.1038/cmi.2014.86

Figure 2.

Figure 2

Effects of rIpaB and rIpaB+rGroEL (co-administration) immunization on antibody levels in mice. Groups of female BALB/c mice (n=6) were immunized i.n. with 40 µg/mouse of recombinant GroEL alone (adjuvant control)/rIpaB alone/rIpaB+rGroEL. Subsequent booster doses were given on the seventh and twenty-eighth days. The control group of mice (n=6) was immunized with sterile PBS. Mice were killed 1 week after the last booster, and sera were collected to estimate the antibody titers by ELISA. (a) Serum IgG, (b) Serum IgA, (c) IgG1 and IgG2a and (d) BALF IgA antibody titers. No detectable IgG1/IgG2a isotope antibodies were observed in the control group. The sera samples which gave an o.d. value >0.3 were measured for scoring. Data were represented as the mean±s.d. P<0.001, a vs. Control, b vs. GroEL, c vs. IpaB. P<0.001, # vs. GroEL, * vs. IpaB. BALF, BAL fluid; ELISA, enzyme-linked immunosorbent assay; PBS, phosphate-buffered saline.