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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Clin Sci (Lond). 2015 Aug;129(3):217–234. doi: 10.1042/CS20140714

Figure 3. Activation of eNOS in PECAM-1 +/+ and PECAM-1 −/− bEND cells.

Figure 3

(A) NO production level in PECAM-1 +/+ and PECAM-1 −/− bEND cells were measured by DAF-FM assay as previously described [185]. (B) Phosphorylation level of Ser1177, Thr495, and Ser113 sites of eNOS were analyzed by Western blot analysis as previously described [185]. All antibodies were from Cell Signaling (Boston, MA). Anti-PECAM-1 was from R&D Systems (Minneapolis, MN) and anti-eNOS was from Santa Cruz (Santa Cruz, CA). Please note diminished production of NO in PECAM-1−/− bEND cells perhaps as a result of increased Ser113 and Thr495 phosphorylation despite similar expression of eNOS and Ser1177 phosphorylation as PECAM-1+/+ bEND cells. (S.Park, CM Sorenson and N. Sheibani, unpublished work)