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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Clin Sci (Lond). 2015 Aug;129(3):217–234. doi: 10.1042/CS20140714

Figure 6. PECAM-1 isoform specific interaction with eNOS and the effect of PECAM-1 expression on NO production.

Figure 6

(A) The interaction between PECAM-1 isoforms and eNOS was assessed by immunoprecipitation (IP) analysis of lysates prepared from PECAM-1 −/− bEND cells expressing a specific isoform of PECAM-1under normal cell culture condition. The amount of total protein for lysates was 1/20 of that applied for IP analysis. (B) Intracellular NO levels in PECAM-1 −/− bEND cells expressing a specific isoform of PECAM-1 was measured using the DAF-FM assay as previously described [185]. Please note that all the PECAM-1 isoforms interacted with PECAM-1. The isoforms lacking exona 14, 15 or both showed higher levels of eNOS compared to full length PECAM-1 or wild type cells. However, expression of none of these isoforms resulted in activation of eNOS and NO production as seen in wild type cells. Thus, simple expression of PECAM-1 and its interaction with eNOS is not sufficient to restore NO levels in null cells. (S.Park, CM Sorenson and N. Sheibani, unpublished work)