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Subcellular localization of TaADF3 in onion cells. Green fluorescent protein (GFP) (A–C) or TaADF3-GFP fusion protein (D–F) was transiently expressed in onion epidermal cells under the control of the cauliflower mosaic virus (CaMV) 35S promoter by particle bombardment. The green fluorescence was observed under a confocal microscope and imaged using a 488-laser excitation light source (A,D). The corresponding cell morphology photos were taken under bright field (B,E). Bar, 50 μm.
