Figure 8.

Enhanced wheat defense response in TaADF3-knockdown plants attacked by virulent Pst CYR31. The amount of H₂O₂ production was measured by calculating the DAB staining area at each infection site using the DP-BSW software (A,B). The area of autofluorescence was measured to determine the necrotic cell death (C). H₂O₂ produced in the guard cells (GC) and the attacked mesophyll cells (MC) was calculated. All results were obtained from 50 infection sites. (D) The expression profiles of three pathogenesis-related proteins were assessed in TaADF3-knockdown plants compared with the mock control plants. The data were normalized to the wheat TaEF-1α gene. Three independent biological replications were performed. Asterisks indicate a significant difference (P < 0.05) from BSMV:00 using Student's t-test.